IMAC Resins

The IMAC Singarose FF are metal chelating affinity chromatography media prepared by coupling nitrilotriacetic acid (NTA) to 6% highly cross-linked agarose. These media, which are not pre-chelated with metal ions, can provide four coordination sites for metal ion chelation, resulting in a more stable binding of the chelated metal ions and allowing them to withstand certain concentrations of reducing agents. After users chelate metal ions such as Cu(2+), Zn(2+), Ni(2+), Co(2+), etc., these media can be used for the separation and purification of recombinant proteins and natural proteins.

Product Type Prepacked columns, Resins

Pack Size 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L

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Product	IMAC Singarose FF
Features	More stable binding of the chelated metal ions and allowing them to withstand certain concentrations of reducing agents
Application	The purification of proteins tagged with histidine-tag
Matrix	6% highly cross-linked agarose
Particle size	90 μm
Functional group	Nitrilotriacetic acid (NTA)
Metal ion loading capacity	20-25 μmol Ni²⁺/mL resin
Binding capacity	When coupling Ni ions，~40mg His-tagged protein/mL resin
Flow velocity	＜250 cm/h
Max. pressure	＜0.3 MPa
pH stability	3-12(operational), 2-14(CIP, short term，when removing metal ions)
Chemical stability	40℃ for1 week：0.01M HCl，0.1M NaOH，8M urea，6M guanidine hydrochloride； 12h：1M NaOH, 70% Acetic acid；1h: 2%SDS；30min：30% Isopropyl alcohol
Storage conditions	20% ethanol; 4-30℃

Pack size	Cat. No.
Pack size	IMAC Singarose FF
1ml XTrap Prepacked columns	HC1040111
5ml XTrap Prepacked columns	HC1040112
5ml XScreen Prepacked columns	HC1040113
10ml	HC1040100
25ml	HC1040101
100ml	HC1040102
500ml	HC1040103
1L	HC1040104
5L	HC1040105
10L	HC1040106