Product

CNBr-activated Singarose 4B

CNBr-activated Singarose 4FF

NHS-activated Singarose 4FF

Expoxy-activated Singarose 6B

Features

The reaction conditions are mild；biomacromolecules can be directly coupled without the need for a coupling spacer arm；they can form multi-point couplings, making the ligand less likely to detach.

Chemically stable；With a 10-carbon atom spacer arm, it reduces the steric hindrance between the ligand and the matrix

Convenience of use, mild coupling conditions, high coupling efficiency, and wide application range.

Application

Used for coupling biomolecules such as proteins and peptides containing primary amines

Used for coupling small proteins or peptides

Used for coupling ligands containing amino, thiol, or hydroxyl groups. It is commonly used for coupling saccharides or other molecules with carbohydrate side chains

Matrix

4% agarose

4% highly cross-linked agarose

6% agarose

Particle size

90 μm

Activating group

Cyanogen bromide

N-Hydroxysuccinimide

1,4-Butanediol diglycidyl ether

Functional group

-NH(2)

-NH(2)，-OH，-SH

Capacity

25-60 mg α-Chymotrypsinogen/mL resin

13-26 mg α-Chymotrypsinogen/mL resin

12-23 μmol/mL resin

19-40 μmol/mL resin

Flow velocity

＜75 cm/h

＜250 cm/h

＜250 cm/h

＜75 cm/h

Max. pressure

＜0.01 MPa

＜0.15 MPa

＜0.15 MPa

＜0.02 MPa

pH stability

3-11(operational), 2-11(CIP, short term)

3-13(operational), 2-13(CIP，short term)

2-14(operational), 2⁓14(CIP，short term)

Storage conditions

Acetone; 2⁓8℃

100% isopropanol

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